Abstract

Acidic polymer complexes isolated from lysozyme digests of Bacillus cereus AHU 1030 cell walls were found to contain mannosamine and glucosamine in addition to typical components of glycerol teichoic acid and glycopeptide. Mild alkali treatment of the complexes gave a glycopeptide linked to a disaccharide which was identified as N -acetylmannosaminyl (1–4) N -acetylglucosamine, while direct treatment of the cell walls with mild acid yielded the same disaccharide linked to teichoic acid. Thus it was concluded that the glycerol teichoic acid chain in the cell walls is linked to the 6-phosphorylated muramic acid residue of peptidoglycan through this disaccharide unit.

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