Abstract

The hydroxyprolyl tri and tetraarabinosides were isolated from a cell wall preparation of cultured tobacco cells by alkali hydrolysis, gel filtration and ion-exchange chromatography. The structures of the two hydroxyproline-arabinosides (Ara3-Hyp and Ara4-Hyp) were determined by analysis of controlled Smith-degradation products, and measurements of PMR spectra and optical rotation to be β-l-Araf-(1→2)-β-l-Araf-(1→2)-β-l-Araf-(1→4)-Hyp and β-l-Araf-(1→3)-β-l-Araf-(1→2)-β-l-Araf-(1→2)-β-l-Araf-(1→4)-Hyp, respectively.

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