Abstract
A genomic library of the sea cucumber Holothuria tubulosa was screened with human and murine histone gene hybridization probes. A recombinant phage carrying an H4 gene was isolated and sequenced. Hybridization analysis of the entire 20 kb phage insert with probes for H1, H2A, H2B and H3 histones was negative except for H2B. This solitary arrangement of the two neighbouring histone H4 and H2B genes is in contrast to the organization of 'cleavage stage' histone genes, which are arranged in tandem quintets of genes encoding the 5 histone classes. Gene organization and sequence features indicate that the two Holothuria genes are the equivalents of a known pair of late H2B and H4 genes which have been described in the genome of sea urchins. This result shows that the simultaneous occurrence of tandem repeats of histone gene quintets and smaller groups of structurally distinct histone gene subtypes is not unique for sea urchins, but also applies to other echinodermata, such as the sea cucumber Holothuria tubulosa.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: DNA sequence : the journal of DNA sequencing and mapping
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
