Abstract
The purpose of this study is to elucidate both the chemical and conformational structure of an unfractionated fucoidan extracted from brown seaweed Turbinaria ornata collected at Nha-trang bay, Vietnam. Electrospray ionization mass spectrometry (ESI-MS) was used for determining the chemical structure and small angle X-ray scattering (SAXS) provided conformational of the structure at the molecular level. The results showed that the fucoidan has a sulfate content of 25.6% and is mainly composed of fucose and galactose residues (Fuc:Gal ≈ 3:1). ESIMS analysis suggested that the fucoidan has a backbone of 3-linked α-l-Fucp residues with branches, →4)-Galp(1→ at C-4 of the fucan chain. Sulfate groups are attached mostly at C-2 and sometimes at C-4 of both fucose and galactose residues. A molecular model of the fucoidan was built based on obtained chemical structure and scattering curves estimated from molecular model and observed SAXS measurement were fitted. The results indicated that fucoidan under study has a rod-like bulky chain conformation.
Highlights
Fucoidans are sulfated polysaccharides derived from marine brown seaweed
Our study aims to elucidate the structure of the fucoidan extracted from brown seaweed Turbinaria ornata collected at Nha-trang bay, Vietnam
A fucoidan isolated from the brown seaweed Turbinaria ornata had high sulfate content and had very simple monosaccharide composition containing mainly fucose and galactose with ratio
Summary
Fucoidans are sulfated polysaccharides derived from marine brown seaweed. The elucidations of the molecular structure, chemical structure and conformation can expand the application of a particular polysaccharide. With the development of high-resolution instrumental processes, such as scattering techniques (i.e., light scattering, X-ray and neutron scattering), it is possible to study the conformation of a polysaccharide at the molecular level. Small Angle X-ray Scattering (SAXS) is a powerful technique that can provide additional structural information of high-resolution structures, and determine the conformation of molecule in solution [15,16]. Our study aims to elucidate the structure of the fucoidan extracted from brown seaweed Turbinaria ornata collected at Nha-trang bay, Vietnam. ESI-MS were used to determine chemical structure and SAXS was employed to elucidate conformational structure at molecular level of the fucoidan. SAXS measurement were compared in order to get useful information about structure of the fucoidan
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