Abstract
A variety of dermatan sulfate samples have been analyzed by the cetylpyridinium chloride-cellulose microcolumn technique before and after treatment elution with testicular hyaluronidase. It was found that “neutral magnesium chloride elution profiles” reflected the molecular-size polydispersity of the material obtained after hyaluronidase degradation. However, “acid magnesium chloride elution profiles” were not influenced by the molecular size to any large extent; instead, the proportions of l-iduronic and d-glucuronic acid as well as the sulfate content seemed to be the principal parameters involved in these elution profiles. Barium acetate-ethanol cellulose elution profiles, finally, were found to separate various dermatan sulfate samples almost entirely according to uronic acid composition. Thus, by comparing elution profiles of unknown samples to those of well-characterized dermatan sulfate polymers, sufficient data were obtained to permit a formulation of the general hybrid properties of beef-aorta and sclera dermatan sulfate as well as of the dermatan sulfate obtained from human knee-joint capsule.
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