Abstract
The structure of a new crystal form of deoxyhemoglobin A grown from polyethylene glycol solutions has been determined at 3·5 A resolution. The molecular orientations and positions were found by means of rotation and translation functions using the squared molecular transform of horse deoxyhemoglobin. Phases were calculated using atomic co-ordinates previously determined for deoxyhemoglobin A grown in another crystalline form. A difference Fourier synthesis showed minor structural differences near intermolecular contacts, the heme groups, and the β 1 carboxyl terminus. Some of these differences may be caused by the different crystalline environment; others may be due to errors in the analytical method. These apparent structural differences will be useful for interpreting results of a similar analysis of deoxyhemoglobin S crystals grown from the same solvent.
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