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Abstract
Isolated metaphase chromosomes of Chinese hamster observed under the scanning electron microscope appear to have a distinct rough surface composed of chromonemata. The chromonema is a fine filament coated with nucleoprotein, which can be easily removed by hypotonic sodium citrate solution. The centromere, a narrowed, tight portion of the chromosome, is also multistranded. No membrane has been seen in metaphase chromosomes.
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