Abstract

Some observations of the structure and development of cell organelles and cell wall have been included in the early experimental works on tissue cultures of trees (2, 3, 17). Differentiation of tracheary and sieve elements was the main aim in several light microscopic studies. The importance of carbohydrate level as well as the role of auxin in cytodifferentiation was determined using callus cultures of Syringa vulgaris (47, 48). Sucrose which was used as sugar in these experiments was favourable for sieve element development at a rather high concentration (4–5%). The same was the case in tissue cultures of Camellia (19). Raffinose and lactose, however, were the most effective carbohydrate sources in the induction of vascularized nodules in Sequoia callus (3).KeywordsSuspension CultureCallus CultureRough Endoplasmic ReticulumCallus LineSieve ElementThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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