Abstract
Replicating λ DNA molecules can be isolated from Escherichia coli shortly after phage infection. During the first round of replication λ DNA has a double-branched circular structure and frequently single-strand connections are observed at the branch points. A study has been made of the single-strand connections to branch points to obtain information about the molecular fine structure at the growing point during replication. The results are consistent with models which allow replication along both DNA strands but which require DNA synthesis only in the 5′ to 3′ direction. A model is presented which requires two sites of DNA synthesis to be associated with each branch point.
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