Abstract
A polysaccharide was isolated by GPC after mild acid treatment of the lipopolysaccharide of Vibrio vulnificus CECT4602 and found to contain l-Rha, d-Glc pNAc and 2-acetamido-2,3,6-trideoxy-3-(3-hydroxybutanoylamino)- l-mannose ( l-RhaNAc3NHb). GLC analysis of the trifluoroacetylated ( S)-2-octyl esters derived by full acid hydrolysis of the polysaccharide showed that ∼80% of the 3-hydroxybutanoic acid has the S configuration and ∼20% the R configuration. The following structure of the polysaccharide was established by 1H and 13C NMR spectroscopies, including 2D ROESY and 1H/ 13C HMBC experiments: ▪
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