Abstract
The structure of the extracellular polysaccharide (EPS) produced by the Rhizobium sp. B strain isolated from atypical nodules on alfalfa has been determined using a combination of chemical and physical techniques (methylation analysis, high pH-anion exchange chromatography (HPAEC), mass spectrometry and 1-D and 2- D NMR spectroscopy). As opposed to the EPS from other strains of Rhizobium, the EPS from the sp. B strain contains d-Glc together with l-Rha and 2-deoxy- d- arabino-hexuronic acid. It is a polymer of a repeating unit having the following structure: →4)- β- d - Glcp-(1 →4)- α- l - Rhap-(1 →3)- β- d - Glcp-(1 →4)-2- deoxy- β- d - Glcp A-(1 → The polysaccharide also contains 0.6 O-acetyl groups per sugar which have not been located.
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