Abstract
Mycoplasma fermentans is thought to be a pathogen of rheumatoid arthritis or cofactor of AIDS. A novel phosphocholine-containing glycoglycerophospholipid named GGPL-I was isolated from a M. fermentans-infected human helper T-cell culture. It was revealed that GGPL-I is a lipid component of the M. fermentans and a major immunological determinant. The GGPL-I was purified by DEAE-Sephadex column chromatography and repeated Iatrobeads column chromatography. The purified glycophospholipid was subjected to structural characterization by thin-layer chromatography, Fourier-transform infrared spectrometry, liquid secondary ion mass spectrometry, and nuclear magnetic resonance spectroscopy. Its structure was determined to be as follows: 6'-O-phosphocholine-alpha-glucopyranosyl-(1'-3)-1,2-diacyl-sn-glycerol. This glycoglycerophospholipid is unique in containing phosphocholine, which is attached to C-6 of glucose. The stereospecific numbering (sn) of naturally occurring GGPL-I was determined through comparison with chemically synthesized compounds.
Highlights
The GGPL-I was put-he study, we detected the occurrenceof several alkaline-labile rified by DFXE-Sephadex column chromatography and glycophospholipids
The stereospecific numbering of We thought that the GGPL-I could be a key substance for naturallyoccurring GGPL-Iwasdeterminedthrough elucidation of the pathogenic roles of M. fermentans
M. fermentans, which is frequently isolated from the synovial fluidof rheumatoid arthritis patients [3] and exhibits agglutinating activity toward erythrocytes, has been suspected of playing some rolein the pathogenesis of rheumatoid arthritis [4]
Summary
GGPL-I is a lipid component of thMe. fermentans anda sphingolipids in the transformed cells[13].During the courseof major immunological determinant. Isolation of GGPL-I-During the course of lipid analysis of Fig. 2a shows the elution profile of the neutrallipid fraction human T-cells [13], we found several slow migrating spots on from a n Iatrobeads column. C, assignment of the product ions GGPL-I on positive MSMS spectrum of rnlz 896.
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