Abstract
The O-specific polysaccharide of Proteus penneri strain 34 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, and H-detected 1H, 13C HMQC experiments. The following structure was established, which is unique among the known structures of Proteus O-antigens: →4)- β- d-Glc p-(1⃗3)- β- d-Gal pNAc-(1⃗4)- β- d-Gal pNAc-(1⃗4)- β- d-Gal p-(1⃗. Accordingly, no cross-reaction was observed between P. penneri 34 O-antiserum and O-antigens of other Proteus strains. Therefore, the strain studied should belong to a new Proteus serogroup O65.
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