Structure of a hinge joint with textured sliding surfaces in terrestrial isopods (Crustacea: Isopoda: Oniscidea)

Abstract

BackgroundThe study of joints in terrestrial arthropods can provide insights into the evolutionary optimization of contacting surfaces that slide without lubrication. This work reports on the structure of the joint between the propodus and the dactylus in terrestrial isopods, the most successful group of crustaceans on land, focusing on the woodlouse Porcellio scaber.MethodsThe joints were studied using fluorescence microscopy, 3D reconstruction, scanning electron microscopy and transmission electron microscopy. The obtained results were functionally interpreted using high-speed video recordings by analyzing the use of the joint during locomotion.ResultsIn the joint, which allows the dactylus to move in a single plain, a semicircular process on the propodus fits into a groove on the dactylus and guides its movement. The sliding surfaces of the propodal process are textured in the form of parallel epicuticular ridges a few hundred nanometers thick. This texturing is selective: while the less heavily loaded surfaces are textured, the surfaces that support the isopod during standing and walking are smooth. In contrast, the groove on the dactylus is completely smooth. We found a similar surface texture in several other species of terrestrial isopods and one aquatic isopod.ConclusionsThe selective texturing of the joint may reduce wear by eliminating small particles. This effect of the ridges was confirmed using electron microscopy. The absence of ridges on heavily loaded surfaces may enhance the dissipation of forces in these regions.