Abstract
Last instar larvae of the insect armyworm, Pseudaletia separata, parasitized with the parasitoid wasp, Apanteles kariyai, do not initiate metamorphosis and, ultimately, the wasp larvae emerge from the host larvae about 10 days after parasitization (Tanaka, T., Agui, N., and Hiruma, K. (1987) Gen. Comp. Endocrinol. 67, 364-374). It is necessary for the parasitoid wasp to perturb the armyworm's endocrinological processes that control normal metamorphosis from larvae to pupae. This endocrinological perturbation allows the parasitoid to complete its larval growth before emerging from the host larvae. It is obligatory for the parasitoid larvae to emerge while the host is still in a larval stage because the sclerotized pupal cuticle is impenetrable for the parasitoid larvae. A growth-blocking peptide with repressive activity against juvenile hormone esterase has been proven to exist in the parasitized host larval plasma (Hayakawa, Y. (1990) J. Biol. Chem. 265, 10813-10816). Here, I describe the detailed structure of this peptide and also the corresponding synthetic peptide to confirm this structure.
Highlights
Chemi~als-[lO-~H]JHI (15.5 Ci/mmol) was purchased from Du parasitoid larvae to emerge while the host is still in a Pont
Peptides were synthesized by solid phase procedures using a larval stage because the sclerotized pupal cuticle is impenetrable for the parasitoid larvae
Bioassay and EnzymeAssay of Peptide-The purified or synthetic peptide was diluted with Ringer’s solution tothe desired concentrationnd injected into unparasitized last instar larvae once at the indicated day(s)
Summary
A growthblocking peptide with repressive activity against juvenile hormone esterase has been proven to exist in the parasitized host larval plasma Bioassay and EnzymeAssay of Peptide-The purified or synthetic peptide was diluted with Ringer’s solution tothe desired concentration (final volume, 5 ~ 1a)nd injected into unparasitized last instar larvae once at the indicated day(s).
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