Abstract

O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Pseudoalteromonas tetraodonis type strain IAM 14160 T and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, 1H, 13C HMQC and HMBC experiments. The polysaccharide was found to consist of hexasaccharide repeating units containing one residue each of d-Gal, d-GlcA, d-GalNAc and d-GlcNAc and two residues of 3,6-dideoxy- l- xylo-hexose (colitose, Col) and having the following structure: In common with the polysaccharides of some other bacteria, the polysaccharide studied contains a tetrasaccharide fragment α-Col p-(1→2)-β- d-Gal p-(1→3)-[α-Col p-(1→4)]-β- d-Glc pNAc, which is a colitose (‘3-deoxy- l-fucose’) analogue of the Lewis b blood group antigenic determinant.

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