Structure of 3'-downstream segment of the human smooth muscle (aortic-type) α-actin-encoding gene and isolation of the specific DNA probe

Abstract

We isolated the 3'-downstream part of the human aortic smooth muscle α-actin (SMαA)-encoding gene and determined the nucleotide sequence, including the ninth (last) exon and 3'-untranslated (UT) region. From the comparison of the human 3'-UT region with rat and chicken 3'-UT regions, its homology is lower than those in 3'-UT regions of other actin isoforms such as cardiac a-actin and cytoskeletal β-actin. Therefore, by using the 3'-UT region of the human SMαA gene as an actin isoform-specific probe, this gene was detected as a single copy only in the human genome, which expressed the 1.7-kb RNA transcript in an aortic tissue-specific manner.