Abstract
For the primary structure analysis of synthetic human galanin and several of its segments, the combination of the fast atom bombardment mapping and the tandem mass spectrometric method was applied. Multistep enzymic digestion performed in situ, i.e. directly on the probe tip and the subsequent high and low energy collision induced dissociation of the resulting hydrolytic products led to sufficient sequence information to elucidate and confirm the structure of the synthetic galanin derivatives.
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