Abstract
Natural products from the genus Euphorbia show attention-attracting activities, such as anticancer activity. In this article, classical isolation and structure identification were used in a study on Caper Euphorbia Seed. Subsequently, MTT and wound healing assays, flow cytometry, western blotting, Hoechst 33258 staining and fluorescence microscopy examination were applied to investigate the anticancer activity of the obtained compounds. In a result, lathyrol-3-phenyl- acetate-5,15-diacetate (deoxy Euphorbia factor L1, DEFL1) was isolated from Caper Euphorbia Seed. Moreover, the NMR signals were totally assigned. DEFL1 showed potent inhibition against lung cancer A549 cells, with an IC50 value of 17.51 ± 0.85 μM. Furthermore, DEFL1 suppressed wound healing of A549 cells in a concentration-dependent manner. Mechanically, DEFL1 induced apoptosis, with involvement of an increase of reactive oxygen species (ROS), decrease of mitochondrial membrane potential (ΔΨm), release of cytochrome c, activity raise of caspase-9 and 3. Characteristic features of apoptosis were observed by fluorescence microscopy. In summary, DEFL1 inhibited growth and induced apoptosis in lung cancer A549 cells via a mitochondrial pathway.
Highlights
Cancer has become the increasing public health problem and the major threat to human health for its high morbidity and mortality [1,2,3]
As part of ongoing research, we have exhaustively investigated Caper Euphorbia Seed, examining the chemical isolation and structure identification of its components and their examining the chemical isolation and structure identification of its components and their pharmacological pharmacological aspects [20,21,22,23]
Lathyrol-3-phenylacetate-5,15-diacetate
Summary
Cancer has become the increasing public health problem and the major threat to human health for its high morbidity and mortality [1,2,3]. As part of ongoing research, we have exhaustively investigated Caper Euphorbia Seed, examining the chemical isolation and structure identification of its components and their examining the chemical isolation and structure identification of its components and their pharmacological pharmacological aspects [20,21,22,23]. During these studies, lathyrol-3-phenylacetate-5,15-diacetate aspects [20,21,22,23]. L1, DEFL1) was isolated from Caper Euphorbia Seed.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
