Structure elucidation and gene cluster annotation of the O-antigen of Pseudomonas veronii SHC-8-1 containing 2-acetamido-2,4,6-trideoxy-4-(3,5-dihydroxyhexanoylamino)-d-glucose

Andrei V Perepelov,Andrei V Filatov,Alexander S Shashkov,Denis S Grouzdev,Tamara L Babich,Nadezhda M Popova,Alexey V Safonov

doi:10.1016/j.carres.2021.108306

Structure elucidation and gene cluster annotation of the O-antigen of Pseudomonas veronii SHC-8-1 containing 2-acetamido-2,4,6-trideoxy-4-(3,5-dihydroxyhexanoylamino)-d-glucose

Andrei V Perepelov, Andrei V Filatov + Show 5 more

https://doi.org/10.1016/j.carres.2021.108306

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Journal: Carbohydrate research

Publication Date: Apr 5, 2021

Affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Frumkin Institute of Physical Chemistry and Electrochemistry

#OPS Structure #2D NMR Spectroscopy + Show 8 more

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Abstract

O-polysaccharide (O-antigen, OPS) was isolated from the lipopolysaccharide of Pseudomonas veronii SHC-8-1 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: [Display omitted] where QuipNAc4N(dHh) is 2,4-diamino-2,4,6-trideoxy-dglucose (Bacillosamine) in which N-2 is acetylated and N-4 is acylated with 3,5-dihydroxyhexanoic acid (dHh). The O-antigen gene cluster of Pseudomonas veronii SHC-8-1 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the OPS structure.

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