Abstract

Investigation of the single plant source bee glue type originating from Southern Australia resulted in the isolation and structure elucidation of major serrulatane diterpenes, novel 7,8,18-trihydroxyserrulat-14-ene (1), along with its oxidized product, 5,18-epoxyserrulat-14-en-7,8-dione (3) and known (18RS)-5,18-epoxyserrulat-14-en-8,18-diol (2). Exploration into the botanical origin revealed Myoporum insulare R. Br, as the plant source of the bee glue materials. This discovery was made through comparative analysis of the myoporum bee glue samples collected from the beehives, analyses of plant resinous exudate, and resin carried on the hind legs of bees foraging for bee glue.

Highlights

  • Bee glue (Propolis) is a resinous substance collected by worker honey bees, Apis mellifera, from specific plants and transported to the hives where it is mixed by bees with beeswax to form strongly adhesive material with antiseptic properties to aid in defense and for maintenance of hygiene in the hive [1,2,3]

  • Considering the unique flora in Australia, only a small number of studies have been done on the Australian bee glue compared with the numerous studies done in other parts of the world

  • There are a number of reports regarding diterpenes obtained from propolis around the world, there there are a number of reports regarding diterpenes obtained from propolis around the world, there is is no report of propolis comprising diterpenoids from Australia

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Summary

Introduction

Bee glue (Propolis) is a resinous substance collected by worker honey bees, Apis mellifera, from specific plants and transported to the hives where it is mixed by bees with beeswax to form strongly adhesive material with antiseptic properties to aid in defense and for maintenance of hygiene in the hive [1,2,3]. The chemical composition of the propolis depends on its plant source which varies with geographical location. The chemical groups of compounds identified in propolis samples include: terpenes, lignans, flavonoids, chalcones, stilbenes, cinnamates, aromatic acids and esters, aliphatic acids and esters, benzophenones, benzofurans and sugars [10,11]. Due to its unique flora, the Southern part of Australia (Kangaroo Island) has been recognized as an area rich in bees’

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