Abstract
A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, β-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible.
Highlights
Ganoderma species, including Ganoderma lucidum (Reishi in Japan or Ling-Zhi in China), has been used for a long time in China to prevent and treat various humanMolecules 2008, 13 diseases
The ground fruiting body of G. lucidum was extracted by hot water to release polysaccharides and the crude polysaccharides were further hydrolyzed by a mild acid degradation (2M trifluoroacetic acid (TFA), 50 oC, 2 hrs) to obtain a low molecular weight and water soluble polysaccharide fraction
In order to investigate the sugar composition of G. lucidum polysaccharide, this polysaccharide fraction was hydrolyzed into monosaccharides by acidic hydrolysis (4M TFA, 121 oC, 6 hrs) and high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used to measure its sugar composition
Summary
Ganoderma species (a group of medicinal fungi), including Ganoderma lucidum (Reishi in Japan or Ling-Zhi in China), has been used for a long time in China to prevent and treat various humanMolecules 2008, 13 diseases. Comparisons of molecular weight and structural information are made between the G. lucidum polysaccharides and glucan standards from dextran, curdlan and starch using We have analyzed non-derivatized G. lucidum polysaccharides, and the permethylated derivatized β-glucan.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
