Abstract
The mica supported Langmuir-Blodgett (LB) monolayers composed of 1,2‑dipalmitoyl‐sn‑glycero‑3‑phosphocholine (DPPC) and 1,2‑dioleoyl‑sn‑glycero‑3‑phosphocholine (DOPC) were used to investigate the effect of cholesterol (Chol) or lauryl gallate (LG) on physicochemical properties of the phospholipid DPPC/DOPC monomolecular film treated as a model leaflet of the bilayer structured membrane. First the surface topography studies of these systems were carried out by means of atomic force microscopy (AFM). Then wettability was investigated via the contact angle measurements and the surface free energy determination. Finally, the time-of-flight secondary ion mass spectrometry (TOF-SIMS) was applied for the surface chemical analysis. Wettability of the monolayers was found to be closely related to the surface chemistry, molecular packing and ordering, miscibility and co-localization of molecules rather than nanoscale roughness. The comparison of the surface polarity determined from the values of contact angle and secondary ion signal intensity provided consistent results. The correlation between the changes of the electron-donor parameter and the 184/57 ratio revealed significance of using the latter quantity as a polarity indicator. The carried out studies show preferential association of components providing a framework for understanding the mechanism of heterogeneities formation within the membrane which reflect the specialization of various regions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
