Abstract

The zebrafish gene for translation elongation factor 1 α (EF1 α) was isolated from a phage Lambda genomic library and sequence and structure determined. One gene copy of EF1 α per haploid set of chromosomes was found and no processed pseudogenes. A highly active promoter region was localized to a 277 bp PstI/ PvuII fragment beginning 240 bp upstream from the tsp, but no transcription enhancing, or silencing activity was observed within 1 kbp upstream, or downstream from the promoter. Expression of EF1 α appears to be developmentally regulated.

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