Abstract
Two major classes of defective herpes simplex virus type 1 ANG (HSV-1 ANG) DNA have been shown to accumulate in independent serial virus passages at high multiplicity of infection. Both types of the DNA’s consist of tandem repeats of small portions of the viral genome and are resistant to Hind III and Hpa I restriction enzyme cleavage. One species of defective DNA — dDNA1 — is not cleaved by Eco RI, whereas the other species has one Eco RI cleavage site in its repeats.
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