Structure and partial protein profiles of the peritrophic membrane (PM) from the gut of the shrimp Litopenaeus vannamei

Abstract

Peritrophic membrane (PM) is a non-cellular structure surrounding the food bolus in invertebrate's midgut. In this study, the shrimp Litopenaeus vannamei was found continuously secreting a tube-like PM enclosing the fecal pellets. The PM structure was membranous in this penaeid shrimp which was similar to that in Sicyonia ingentis studied and was primarily composed of chitin and proteins. Chitin was detected along the whole PM. By using the approach of gel electrophoresis and mass spectrometry of tryptic peptides, the most extracted proteins from the shrimp PMs were identified mainly including digestion-related, immune-related, antioxidant proteins and proteins related to PM structure. This suggests that PM may participate in modulating its permeability and immobilizating the digestive enzymes, actively protect the gut from pathogen contact, and play an important role in the gut immune system.