Structure and organization of the subepithelial microvasculature in the canine nasal mucosa

Abstract

To quantitatively characterize the epithelial microvasculature in the transitional nasal mucosa of the dog, septal, dorsal conchal, and ventral conchal tissues were prepared for light microscopy. Subsequent to the drilling of orientation holes perpendicularly into the epithelium, serial 1.0-μm sections were cut parallel to the epithelial plane. A computerized data acquisition system was used in which the sections were aligned and the lumen/wall interfaces of capillaries digitized. Information stored in the program included the position of capillaries, their diameter, their major axis length, and the angle between their major axis and the X axis of the coordinate system. The intraepithelial capillary loops constituted 9% of the epithelial volume, and had a surface area of 31.18 mm 2/mm 3 of epithelium, and a length of 1055 mm/mm 3 of tissue. Their diameters ranged from 9.1 to 11.3 μm for the three tissues sampled. The distances between afferent and efferent limbs of capillary loops were 4.4, 4.5, and 13.8 μm for nasal septum, alar fold, and dorsal concha, respectively. Additional analyses indicated that capillary loops were neither arranged in rows nor oriented to airflow. Rather than conducting heat to the luminal surface, the primary function of these vessels may be to transport cells and/or cytokines to and from a specialized epithelium, one that is adapted to metabolizing contaminants deposited during breathing.