Abstract
How benzene is metabolized by microbes under anoxic conditions is not fully understood. Here, we studied the degradation pathways in a benzene-mineralizing, nitrate-reducing enrichment culture. Benzene mineralization was dependent on the presence of nitrate and correlated to the enrichment of a Peptococcaceae phylotype only distantly related to known anaerobic benzene degraders of this family. Its relative abundance decreased after benzene mineralization had terminated, while other abundant taxa-Ignavibacteriaceae, Rhodanobacteraceae and Brocadiaceae-slightly increased. Generally, the microbial community remained diverse despite the amendment of benzene as single organic carbon source, suggesting complex trophic interactions between different functional groups. A subunit of the putative anaerobic benzene carboxylase previously detected in Peptococcaceae was identified by metaproteomic analysis suggesting that benzene was activated by carboxylation. Detection of proteins involved in anaerobic ammonium oxidation (anammox) indicates that benzene mineralization was accompanied by anammox, facilitated by nitrite accumulation and the presence of ammonium in the growth medium. The results suggest that benzene was activated by carboxylation and further assimilated by a novel Peptococcaceae phylotype. The results confirm the hypothesis that Peptococcaceae are important anaerobic benzene degraders.
Highlights
Benzene is part of petroleum and gasoline and can contaminate soil, sediment and aquifers during oil extraction, production and related industrial activities (Landon & Belitz, 2012)
Benzene is slowly metabolized by microbial consortia under different redox conditions; only a few pure cultures have been far described that are capable of anaerobic benzene mineralization (Coates et al, 2001; Holmes et al, 2011; Kasai et al, 2006)
Putative pathways for nitrate reduction coupled with benzene mineralization
Summary
Benzene is part of petroleum and gasoline and can contaminate soil, sediment and aquifers during oil extraction, production and related industrial activities (Landon & Belitz, 2012). Benzene is slowly metabolized by microbial consortia under different redox conditions; only a few pure cultures have been far described that are capable of anaerobic benzene mineralization (Coates et al, 2001; Holmes et al, 2011; Kasai et al, 2006). It is not fully understood how benzene is activated in the absence of oxygen.
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