Abstract
Ribulose-1, 5-diphosphate carboxylase isolated from autotrophically grown cells of the green algae, Chlorella ellipsoidea, was partially purified. The nature of the enzyme closely resembles the spinach leaf enzyme in its elution patterns on Sephadex gel filtration and DEAE-cellulose column chromatography, migration on starch gel electrophoresis, and the immunological specificity. Chlorella RuDP carboxylase exhibits essentially identical kinetic parameters of the regulatory enzyme with those of spinach enzyme: (a) homotropic effect of NaHCO 3, (b) allosteric activating effect of Mg 2+ with respect to NaHCO 3, and (c) shift of optimum pH by elevating the Mg 2+ concentrations.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
