Abstract
I report on the isolation, structural analysis, and in vivo expression patterns of a fungal calmodulin gene. The gene is intronless and encodes a protein of 148 amino acid residues that is 92% homologous with vertebrate calmodulins. Through S1 nuclease transcript mapping, it was determined that the cloned gene (a) is transcribed in vivo, (b) has a 5'-untranslated region of about 400 nucleotides, and (c) has a 3'-untranslated end of about 300 nucleotides. Southern blot hybridization analysis of the genomic DNA and the cloned gene provide evidence for the existence of only one type of calmodulin gene in the organism. The amino acid sequence deduced from the DNA sequence shows that Achlya klebsiana calmodulin has amino acid substitutions that are a mix of those seen in calmodulins from invertebrates such as Drosophila and trypanosome when compared to mammalian calmodulins. Not surprisingly, it has less resemblance to calmodulins from Saccharomyces and Dictyostelium.
Highlights
(25), and sugars (26),and theprocess of sporulation (27) were all shown to be dependent on the availability of Ca2+.Ca2+, must play a pivotal role in cellular homeostasis of Achlya
The powder was pivotal role in the regulation of cellular homeostasis (1).These diverse cellular activities of Ca2+are mediated by cytoplasmic Ca2+receptors of which calmodulin is the major one in non
Preparation of Phagemid MIS/Calmodulin DNA RecombinantsPhagemids M13+ and M13- (Stratagene, San Diego,CA)were linearized with ClaI and ligated independently to 2.0- and 1.7-kb ClaI fragments recovered from a recombinant clone isolated from the genome, and known to contain the fungal calmodulin gene
Summary
(25), and sugars (26),and theprocess of sporulation (27) were all shown to be dependent on the availability of Ca2+.Ca2+, must play a pivotal role in cellular homeostasis of Achlya. RNA on Hybond was probed with a 3.8-kb Sal[1] DNA fragment containing the full Achlya calmodulin gene.
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