Abstract
We determined the complete sequences of the mitochondrial DNA control regions in Evynnis cardinalis using long polymerase chain reaction(PCR),and aligned them by Clustal X with 9 related Sparidae sequences in GenBank.Several conserved sequence blocks were identified in the mitochondrial DNA control regions,including extended terminal associated sequences(ETAS),blocks of central domain(CSB-F,CSB-E,CSB-D,CSB-C,CSB-B,CSB-A) and conserved sequence blocks(CSB1,CSB2,CSB3).Taking Paralabrax clathratus and Verasper moseri as outgroups,the molecular phylogenetic trees of Sparidae were constructed based on neighbour-joining and maximum-parsimony methods,which reveal that Sparidae is a monophyletic group,and E.cardinalis should be grouped with Pagrus.Therefore,it is necessary to reevaluate the taxonomic status of Evynnis and Pagrus.
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