Structure and chromosome assignment of human S100 α and β subunit genes

Abstract

The human S-100 alpha and beta subunit genes were isolated from human genomic DNA library. The restriction endonuclease mapping and DNA sequencing analysis revealed that both subunit genes consisted of three exons and two introns. Two Ca2(+)-binding domains were independently encoded by exon 2 and exon 3. Spot-blot hybridization analysis with flow-sorted chromosomes showed that human alpha and beta subunit genes were assigned to chromosome 1 and chromosome 21, respectively.