Structure and bioelectricity of single neurons of Helix pomatia in the intact nervous tissue during epileptic activity: Simultaneous evaluations by confocal microscopy and intracellular recordings of membrane potential changes

Abstract

1. 1. Neuronal shape during epileptic activity was studied with confocal laser scanning microscopy and intracellular recording of identified neuronal individuals in the buccal ganglia of Helixpomatia. Simultaneous observations of single Lucifer Yellow stained fibers and epileptic activity of the same neuron were done. 2. 2. During epileptic activity, the development of several types of morphological changes were observed: local and extended swellings, constrictions, stretching of neuronal processes and release of intracellular material. 3. 3. Morphological alterations did not only occur with epileptic activity but could also appear due to extended laser exposure of fluorescent neuronal processes. 4. 4. Phototoxicity is discussed as a limiting factor for a valid interpretation of the morphological changes observed by confocal microscopy.