Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.

Pingchuan Yuan,Kaoshan Chen,Jun Han,Yuyan Zhou,Fang Fang,Ping Li,Guodong Wang,Wei Hu,Taili Shao

doi:10.3390/molecules24071345

Pingchuan Yuan, Kaoshan Chen + Show 7 more

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https://doi.org/10.3390/molecules24071345

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Abstract

In this study, an extracellular polysaccharide from Alternaria mali Roberts (AMEP) was extracted, and its structure was characterized, in addition to its antitumor activity in vitro. Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios. The linking mode of both AMEP-1 and AMEP-2 is Manp-(1→4) and Glcp-(1→6), and the branched chains are connected to the main chain through O-6. AMEP-2 inhibited the proliferation of BGC-823 cells in a time- and concentration-dependent manner. AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells. Therefore, AMEP-2 shows potential for further development as a novel anti-tumor agent.

Highlights

Cancer remains a serious threat to human life and safety, and the conventional chemotherapy drugs used in cancer treatment have strong cytotoxicity, low selectivity, and induce damage to the host immune system, often resulting in serious infection to exert more damage to patients than the initial disease [1,2,3]
The results show that AMEP-2 could increase the level of oxidative stress in BGC-823 cells and promote in apoptosis
Alternaria mali Roberts strain was isolated from an apple, which was collected from Shandong

Summary

Introduction

Cancer remains a serious threat to human life and safety, and the conventional chemotherapy drugs used in cancer treatment have strong cytotoxicity, low selectivity, and induce damage to the host immune system, often resulting in serious infection to exert more damage to patients than the initial disease [1,2,3]. Further exploration of naturally found polysaccharides can advance the development of new cancer treatments and combination treatments to improve the outcome of chemotherapy and the quality of life of patients

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