Abstract
Abstract This study aimed to investigate that the antioxidant capacity of the peptides Met-Thr-Thr-Asp-Ile (MTTNI) and Met-Thr-Thr-Asp-Leu (MTTNL) purified from shrimp protein hydrolysate (SPH), identified by UPLC-Q-TOF-MS, and the related signaling pathways that inhibit oxidative stress. The results indicated that the inhibition ratios of MTTNI and MTTNL hydroxyl radical scavenging activities were 10.71 ± 3.27% and 8.94 ± 0.97%, respectively, and those of their DPPH radical scavenging activities were 43.21 ± 3.55% and 38.74 ± 2.82%, respectively. The two peptides significantly decreased ROS content in PC12 cells and upregulated SOD activity to the level of the scopolamine group. Moreover, MTTNL and MTTNI could modulate oxidative stress in vitro, and the presence of Leu in the peptide sequence may be the critical factor contributing to stronger antioxidant activity than peptide sequences with Ile. MTTNL western blot analysis showed Leu suppresses Bax, Caspase-3, and p53 expression, as well as BCL-XL overexpression, thus protecting PC12 cells from oxidative stress. Overall, the peptides protected neurons from oxidative damage through the anti-apoptotic pathway.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
