Structural variability and differentiation of niches in the rhizosphere and endosphere bacterial microbiome of moso bamboo (Phyllostachys edulis)

Zong-Sheng Yuan,Qiu-Liang Huang,Fang Liu,Guo-Fang Zhang,Zhen-Yu Liu,Hui Pan

doi:10.1038/s41598-021-80971-9

Abstract

The plant microbiota play a key role in plant productivity, nutrient uptake, resistance to stress and flowering. The flowering of moso bamboo has been a focus of study. The mechanism of flowering is related to nutrient uptake, temperature, hormone balance and regulation of key genes. However, the connection between microbiota of moso bamboo and its flowering is unknown. In this study, samples of rhizosphere soil, rhizomes, roots and leaves of flowering and nonflowering plants were collected, and 16S rRNA amplicon Illumina sequencing was utilized to separate the bacterial communities associated with different flowering stages of moso bamboo. We identified 5442 OTUs, and the number of rhizosphere soil OTUs was much higher than those of other samples. Principal component analysis (PCA) and hierarchical clustering (Bray Curtis dis) analysis revealed that the bacterial microorganisms related to rhizosphere soil and endophytic tissues of moso bamboo differed significantly from those in bulk soil and rhizobacterial and endosphere microbiomes. In addition, the PCA analyses of root and rhizosphere soil revealed different structures of microbial communities between bamboo that is flowering and not flowering. Through the analysis of core microorganisms, it was found that Flavobacterium, Bacillus and Stenotrophomonas played an important role in the absorption of N elements, which may affect the flowering time of moso bamboo. Our results delineate the complex host-microbe interactions of this plant. We also discuss the potential influence of bacterial microbiome in flowering, which can provide a basis for the development and utilization of moso bamboo.

Highlights

The plant microbiota play a key role in plant productivity, nutrient uptake, resistance to stress and flowering
By comparing the different OTU sparse curves of moso bamboo rhizosphere soil and endophytic tissue, we found that the rhizosphere soil showed a uniform sparse curve, and the change in sparse curve was much higher than that of sample of endophytic tissue, compared with that of the leaf tissue (Fig. 1)
These results show that the rhizosphere bacterial community of moso bamboo is highly abundant, and they were consistent with those of p oplar23, tomato[24,25] and other species, indicating that the rhizosphere bacterial microbiome was aggregated

Summary

Introduction

The plant microbiota play a key role in plant productivity, nutrient uptake, resistance to stress and flowering. We evaluate the differentiation of niches of microbiome of bacterial communities associated with the rhizome, roots, leaves, and rhizosphere and bulk soil of flowering and nonflowering moso bamboo using 16S rRNA Illumina sequencing.

Results

Conclusion