Abstract
1. 1. The nuclear protein composition of the sperm consists of a heterogeneous mixture of histones and protamine-like components. 2. 2. Independently of their somatic or sperm specific origin, the histones are always present in their full complement: H2A, H2B, H3, H4, including H1, and exhibit the same stoichiometry as that observed in the somatic-type chromatin. 3. 3. One step fractionation procedure is described for the isolation and purification of most of the protamine-like components. 4. 4. Although the number of the protamine-like components and their physical parameters are variable between and within the different orders and families studied, nevertheless, the chemical composition is very similar for most of them.
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