Abstract
Acidic glycosaminoglycans (AGAG) were extracted from whole human kidney tissues of different aged subjects and were purified by precipitation with cetylpyridinium chloride and by an ion exchange resin column chromatography. The prepared AGAG were individually digested with chondroitinase-AC and they were further digested with chondroitinase-ABC. The resulting unsaturated disaccharides and the undigested AGAG were identified by paper chromatography, electrophoresis, and other chemical analyses. The AGAG, which were resistant to chondroitinase-AC, were mainly heparan sulfates and their proportion to total AGAG appeared to decrease with the advance of age. Dermatan sulfate plus oversulfated dermatan sulfate were composed of one-fifth to one-third of the AGAG resistant to chondroitinase-AC, and their proportion tended to increase with the advance of age.
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