Abstract
Abstract The water-soluble polysaccharide isolated from hot aqueous extract of the fruit bodies of somatic hybrid mushroom ( pfle 1q ) obtained through intergenic protoplast fusion between Pleurotus florida and Lentinula edodes consists of d -galactose and d -mannose in a molar ratio of nearly 2:1. The polysaccharide showed immunoenhancing activity by stimulating the macrophage, splenocyte, and thymocyte. Based on the results of acid hydrolysis, methylation analysis, periodate oxidation, and NMR experiments ( 1 H, 13 C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC and HMBC) the repeating unit of the purified polysaccharide was established as
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