Abstract
The O-specific polysaccharide of H. alvei strain PCM 1185 contains d-glucose, d-glucuronic acid, 2-acetamido-2-deoxy- d-glucose, and 3,6- dideoxy-3-[(R)-3- hydroxybutyramido]- d-glucose (Qui3NAcyl) in the ratios 2:1:1:1, as well as O-acetyl groups. On the basis of sugar and methylation analyses of the polysaccharide before and after chemical modifications ( O-deacetylation, carboxyl reduction, Smith degradation), as well as 1H and 13C NMR spectroscopy, including 1D sequential, selective spin-decoupling, 2D homonuclear, and 13C, 1H heteronuclear correlation spectroscopy (COSY), and 2D rotating-frame NOE spectroscopy, it was found that the polysaccharide has a pentasaccharide repeating unit with the following structure: ▪ with O-acetyl groups present in nonstoichiometric amounts, mainly at position 2 of GlcA and position 6 of GlcNAc or lateral Glc. Serological study showed that H. alvei strain PCM 1185 can be placed in a new serotype D and that an O-acetyl group can be a part of its epitope.
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