Abstract
The structure of the O-specific side-chains of the cell-wall lipopolysaccharide of Salmonella typhimurium 395 MS has been investigated. Methylation analyses of the original lipopolysaccharide, of the material obtained on mild hydrolysis of the lipopolysaccharide with acid, and of a product obtained by acetalation of all of the, free hydroxyl groups in the lipopolysaccharide, followed by alkaline deacetylation, have provided the essential information in this study. The mixtures of sugars obtained on acid hydrolysis of the different methylated products were analysed, as alditol acetates, by g.l.c.-mass spectrometry. As a result of these studies, a detailed structure of the repeating unit of these side-chains is presented.
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