Structural studies on a human bladder stone — PMR and IR studies

Abstract

Wide line Proton Magnetic Resonance spectra of a human urinary bladder stone powder at several temperatures (in the range 77 K to 298 K) and its IR spectra (at 77 K and 298 K) were recorded and analysed. The PMR spectrum at 77 K was a broad signal and at 143 K it exhibited two signals, one broad and one narrow superimposed on each other. The line width of the broad signal and its second moment value remained constant up to 298 K and this signal was identified as due to the protons of water molecules in the sample. The narrow signal was attributed to the protons of NH4 group in the sample and reduction in the value of its second moment at higher temperatures has been interpreted as due to the hindered rotation of NH4 group. The IR spectrum at 77 K has shown the presence of O−H... O bonds in the sample.