Abstract
A major glycoprotein of M r = 36 000 has been isolated from lung lavage of normal rabbit and purified to homogeneity by gel chromatography. The glycoprotein was found to contain 4 residues hydroxyproline, 50 residues glycine, 3 residues methionine, 2.6 mol sialic acid, 4.0 mol galactose, 5.0 mol mannose, 0.9 mol fucose and 7.0 mol glucosamine. Cyanogen bromide treatment of the glycoprotein resulted in four peptides with molecular weights of 18 000, 12 000, 5000 and 1000, three of the peptides containing hydroxyproline and two of the peptides containing carbohydrates. Partial NH 2-terminal amino acid sequence analysis on one of the cyanogen bromide peptides ( M r = 18 000) indicated the presence of -Gly-Pro-Hyp-Gly- sequence in the peptide chain, suggesting that collagen-like region(s) may be present in this glycoprotein.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Protein Structure
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
