Structural Studies of the Lipopolysaccharide Isolated from Plesiomonas shigelloides O22:H3 (CNCTC 90/89).

Anna Maciejewska,Czeslaw Lugowski,Jolanta Lukasiewicz,Brygida Bednarczyk

doi:10.3390/ijms21186788

Abstract

Plesiomonas shigelloides is a Gram-negative, rod-shaped bacterium which causes foodborne intestinal infections, including gastroenteritis. It is one of the most frequent causes of travellers’ diarrhoea. Lipopolysaccharide (LPS, endotoxin), an important virulence factor of the species, is in most cases characterised by a smooth character, demonstrated by the presence of all regions, such as lipid A, core oligosaccharide, and O-specific polysaccharide, where the latter part determines O-serotype. P. shigelloides LPS is still a poorly characterised virulence factor considering a “translation” of the particular O-serotype into chemical structure. To date, LPS structure has only been elucidated for 15 strains out of 102 O-serotypes. Structures of the new O-specific polysaccharide and core oligosaccharide of P. shigelloides from the Czechoslovak National Collection of Type Cultures CNCTC 90/89 LPS (O22), investigated by chemical analysis, mass spectrometry, and 1H,13C nuclear magnetic resonance (NMR) spectroscopy, have now been reported. The pentasaccharide repeating unit of the O-specific polysaccharide is built of one d-QuipNAc and is rich in four d-GalpNAcAN residues. Moreover, the new core oligosaccharide shares common features of other P. shigelloides endotoxins, i.e., the lack of phosphate groups and the presence of uronic acids.

Highlights

Plesiomonas shigelloides is the only species in the genus Plesiomonas and belongs to the Enterobacteriaceae family
Extra-intestinal infections caused by P. shigelloides, most notably, meningitidis in neonates, bacteraemia, sepsis, and septic shock, were uncommonly reported for this, but sepsis and meningitidis are related with the serious course and high fatality rate [3,4]
The aim of this study was to extend the knowledge about structural features of P. shigelloides LPS and to investigate the structure of the new O-specific polysaccharide and core oligosaccharide and the linkage between them of the serotype O22 LPS

Summary

Introduction

Plesiomonas shigelloides is the only species in the genus Plesiomonas and belongs to the Enterobacteriaceae family. It is usually isolated from fish, crabs, prawns, mussels, and oysters, as well as birds, amphibians, and mammals. The species is a Gram-negative, oxidise-positive, rod-shaped bacterium which causes intestinal infections associated with consumption of seafood, uncooked food, and contaminated water. To Latin America, the Caribbean, and South and Southeast Asia, is a second major risk factor associated with Plesiomonas infections in humans [1]. Extra-intestinal infections caused by P. shigelloides, most notably, meningitidis in neonates, bacteraemia, sepsis, and septic shock, were uncommonly reported for this, but sepsis and meningitidis are related with the serious course and high fatality rate [3,4]

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