Abstract
The structures of the sugar chains present in two human monoclonal IgM molecules purified from the serum of a patient with Waldenström's macroglobulinemia have been determined. The asparagine-linked sugar chains were liberated as oligosaccharides by hydrazinolysis and labeled by reduction with NaB 3H 4 after N-acetylation. Their structures were studied by serial lectin column chromatography and sequential exoglycosidase digestion in combination with methylation analysis. These two IgM's were shown to contain almost the same sugar chains. The sugar chains were a mixture of a series of high-mannose-type and biantennary complex-type oligosaccharides. The complex-type oligosaccharides contain Manα1 → 6(±GlcNAcβ1 → 4)(Manα1 → 3)Manβ1 → 4GlcNAcβ1 → 4(Fucα1 → 6)GlcNAc as their core and GlcNAcβ1 →, Galβ1 → 4GlcNAcβ1 → and Neu5Acα2 → 6Galβ1 → 4GlcNAcβ1 → groups in their outer chain moieties.
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