Abstract
The interface of a protein molecule that is involved in binding another protein, DNA or RNA has been characterized in terms of the number of unique secondary structural segments (SSSs), made up of stretches of helix, strand and non-regular (NR) regions. On average 10-11 segments define the protein interface in protein-protein (PP) and protein-DNA (PD) complexes, while the number is higher (14) for protein-RNA (PR) complexes. While the length of helical segments in PP interaction increases with the interface area, this is not the case in PD and PR complexes. The propensities of residues to occur in the three types of secondary structural elements (SSEs) in the interface relative to the corresponding elements in the protein tertiary structures have been calculated. Arg, Lys, Asn, Tyr, His and Gln are preferred residues in PR complexes; in addition, Ser and Thr are also favoured in PD interfaces.
Highlights
Characterization of protein-protein (PP), protein-DNA (PD) and protein-RNA (PR) interactions is essential for understanding the mechanisms of biological processes on a molecular level
The interface of a protein molecule that is involved in binding another protein, DNA or RNA has been characterized in terms of the number of unique secondary structural segments (SSSs), made up of stretches of helix, strand and non-regular (NR) regions
While the length of helical segments in PP interaction increases with the interface area, this is not the case in PD and PR complexes
Summary
Characterization of protein-protein (PP), protein-DNA (PD) and protein-RNA (PR) interactions is essential for understanding the mechanisms of biological processes on a molecular level. Structural segments and residue propensities in protein-RNA interfaces: Comparison with proteinprotein and protein-DNA complexes
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