Structural responses of differentially adapted Cenchrus setigerus Vahl ecotypes to water deficit

Abstract

Two ecotypes of Cenchrus setigerus Vahl (birdwood grass) were collected from dry mountains surrounding hyper-saline Kalar Kahar Lake and Gatwala artificial forest plantation. The ramets of the two ecotypes were established in pots for 15 days and then subjected to 100% FC (control) or 75% and 50% FC (field capacity) levels. Growth attributes such as plant height, tillers per plant, leaves per plant, shoot fresh and dry weights were severely affected while root length was the only attribute that increased in both ecotypes under moisture stress. Root anatomical attributes like cortical and pith parenchyma, and vascular region thickness increased in both ecotypes under water stress. Root radius increased in the Faisalabad ecotype at 75% FC, while increased in Salt Range ecotype only at 50% FC. Stem cellular region, sclerenchymatous cell area, vascular bundle area and metaxylem area increased significantly under moderate and severe water deficit only in the Faisalabad ecotype. The Salt Range ecotype exhibited intensive sclerification and large vascular tissue at 50% FC. Leaf anatomical attributes like epidermal cell area, bundle sheath thickness, sclerenchyma thickness and its cell area, and, vascular bundles area significantly increased in the Salt Range ecotype with increasing water deficit. The Faisalabad ecotype showed significant increase in vascular bundle area, metaxylem area and adaxial stomatal area. These findings indicated that both C. setigerus ecotypes mainly relied on water conservation and survival rather than maintaining growth and biomass production. The anatomical modifications at root, stem and leaf levels ensured survival of C. setigerus ecotypes in extreme arid conditions. • Growth attributes were severely affected while root length increased under stress • Root radius increased in the Faisalabad ecotype at 75% FC, while at 50% FC in Salt Range ecotype • Stem area, sclerification and vascular bundle area increased only in Faisalabad ecotype • The Salt Range ecotype exhibited intensive sclerification in leaves and large vascular tissue at 50% FC • The Faisalabad ecotype showed an increase in vascular bundle area, metaxylem area and adaxial stomatal area