STRUCTURAL REORGANIZATION OF THE RATS’ SUBMANDIBULAR GLANDS ACINI AFTER THE INFLUENCE OF 1% METHACRYLATE

Abstract

Introduction: Polymeric substances, which are used in medicine, can lead to the development of pathological conditions. Therefore, in toxicological evaluation of polymeric materials for medical purposes the primary task is the creation of the appropriate adequate methods of study. The aim: To studytheacinioftherats'submandibularsalivaryglandsin normalcondition andafter exposureof 1% methacrylate. Material andmethods:Were studied 50 white rats during the influence of 1% methacrylate on the mucosa of the oral cavity. Histological sections of the rats'submandibularsalivary glands in normalcondition, on day 14 and 30 of experiment were studied using the UMPT - 7 ultramicrotome of Sumy PA "Selmi". Results and conclusions:Themorphometric study hasestablishedthat in control group thevaluesof theouterdiameterof thesubmandibularglands' acini, the diameter of the lumen and the height of the acini epithelial cells was 36,27±2,17 µm,9,47±0,63 µm and 14,18±1,05 µm, respectively. Histological specimens showed that the acini cells had a prominent basophilia of the cytoplasm and the basally located nucleus. Numerous large homogeneous granules were located in the apical pole of the cells. After influence of the 1% methacrylat there is an increase in the activity of secretory cells, which is confirmed by an increase in the height of epitheliocytes by 25.04% and is a compensatory metacrylate reaction. The increase in salivation further leads to complete depletion of the secretory apparatus of the seromucosal cells, which is confirmed by a decrease in the outer and inner diameters with a decrease in the height of the mandibular salivary glands acini cells by 24.40%.