Abstract
AbstractThe purpose of the present experiment is to study the different effects of natural steroid molecules (NSM) on the crystallization of cholesterol in model bile and ethanol. Three natural steroids (stigmasterol, β‐sitosterol, and ergosterol) were selected as model molecules. The supernatant and precipitated crystals collected from 95% ethanol and model bile were quantitatively analyzed by high‐performance liquid chromatography‐evaporative light scattering detection (HPLC‐ELSD), powder X‐ray diffraction, and differential scanning calorimetry, as well as scanning electron microscope, were conducted to understand the crystalline properties of a substance. Results show that Stigmasterol and β‐sitosterol can increase the solubility of cholesterol in specific mass ratios under 95% ethanol system, and lower the solubility of cholesterol in model bile. Ergosterol, on the other hand, has no significant effect on cholesterol crystallization. The reason for this phenomenon is that phytosterols are more similar in molecular structure to cholesterol and act as co‐precipitates with cholesterol in both systems.
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