Abstract
The design and screening of nanoparticles for therapeutic applications (nanodrugs) belong to an emerging research area, where surface based analytical techniques are promising tools. This study reports on the interaction of electrostatically assembled nanoparticles, developed for non-invasive administration of human insulin, with cell membrane mimics. Interactions between the nanoparticles and differently charged surface-supported model membranes were studied in real-time with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, in some experiments combined with optical reflectometry. Based on the experimental observations, we conclude that structural rearrangements of the nanoparticles occur upon adsorption to negatively charged lipid membranes. The degree of nanoparticle deformation will have important implications for the induced release of the protein drug load. The presented results provide an example of how a surface-based experimental platform can be used for evaluation of nanosized drug carriers.
Highlights
Nanotechnology based methodologies are important tools for the development of novel drug delivery systems
This study reports on the interaction of electrostatically assembled nanoparticles, developed for non-invasive administration of human insulin, with cell membrane mimics
Interactions between the nanoparticles and differently charged surface-supported model membranes were studied in real-time with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, in some experiments combined with optical reflectometry
Summary
Nanotechnology based methodologies are important tools for the development of novel drug delivery systems. It is often desired to protect drugs before they reach their site of action and to minimize adverse systemic effects by targeting of active substances and their local release in a particular tissue or cell [3,4] In the following, we will focus on studies of a nanoparticulate protein drug formulation and its interaction with surface supported biomimetic membranes These membranes are well-defined model systems, which can be controlled and manipulated at the molecular and nano-level with respect to lipid composition [5], morphology [6], as well as with respect to the incorporation of various membrane bound [7] or membrane associated [8] molecules. The combination of these three signals has been shown to constitute a powerful way of characterizing supported lipid membrane formation and interactions/remodeling of such bilayers [13]
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